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thp 1 blue tm cells  (InvivoGen)


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    Structured Review

    InvivoGen thp 1 blue tm cells
    A, B, E, F) NF-κB activation in HEK (A, E) and <t>THP-1</t> (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.
    Thp 1 Blue Tm Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thp 1 blue tm cells/product/InvivoGen
    Average 90 stars, based on 2 article reviews
    thp 1 blue tm cells - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Lipoglycans Contribute to Innate Immune Detection of Mycobacteria"

    Article Title: Lipoglycans Contribute to Innate Immune Detection of Mycobacteria

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0028476

    A, B, E, F) NF-κB activation in HEK (A, E) and THP-1 (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.
    Figure Legend Snippet: A, B, E, F) NF-κB activation in HEK (A, E) and THP-1 (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.

    Techniques Used: Activation Assay, Derivative Assay, Incubation, Bacteria, Concentration Assay

    A, B, E, F) NF-κB activation in HEK-TLR2 (A, E) and THP-1 (B, F) cells. C, D, G, H) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D, H) cells. Cells were incubated with bacteria at MOI of 1. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001. n.i., not induced.
    Figure Legend Snippet: A, B, E, F) NF-κB activation in HEK-TLR2 (A, E) and THP-1 (B, F) cells. C, D, G, H) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D, H) cells. Cells were incubated with bacteria at MOI of 1. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001. n.i., not induced.

    Techniques Used: Activation Assay, Derivative Assay, Incubation, Bacteria



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    A, B, E, F) NF-κB activation in HEK (A, E) and <t>THP-1</t> (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.
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    A, B, E, F) NF-κB activation in HEK (A, E) and <t>THP-1</t> (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.
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    Image Search Results


    A, B, E, F) NF-κB activation in HEK (A, E) and THP-1 (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.

    Journal: PLoS ONE

    Article Title: Lipoglycans Contribute to Innate Immune Detection of Mycobacteria

    doi: 10.1371/journal.pone.0028476

    Figure Lengend Snippet: A, B, E, F) NF-κB activation in HEK (A, E) and THP-1 (B, F) cells. C, D, G) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D) cells. Cells were incubated with bacteria at MOI of 1. In F and G, cells were pre-incubated for 30 min at 37°C before bacteria addition with various antibodies, anti-TLR2 and isotype controls, at a concentration of 5 µg/ml. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. In A, B, C and D), P value is given vs pMV. n.i., not induced.

    Article Snippet: THP-1-Blue TM cells (Invivogen), derivatives of THP-1 monocyte/macrophage human cells that stably express a NF-κB-inducible reporter system (secreted alkaline phosphatase), were added at 10 5 cells per well in 96-wells plates in HEK-Blue TM Detection medium (for NF-κB activation assay) or differentiated with 20 ng/ml of PMA for 24 h in RPMI 1640 culture medium (Lonza, Verviers, Belgium) (for IL-8 production assay).

    Techniques: Activation Assay, Derivative Assay, Incubation, Bacteria, Concentration Assay

    A, B, E, F) NF-κB activation in HEK-TLR2 (A, E) and THP-1 (B, F) cells. C, D, G, H) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D, H) cells. Cells were incubated with bacteria at MOI of 1. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001. n.i., not induced.

    Journal: PLoS ONE

    Article Title: Lipoglycans Contribute to Innate Immune Detection of Mycobacteria

    doi: 10.1371/journal.pone.0028476

    Figure Lengend Snippet: A, B, E, F) NF-κB activation in HEK-TLR2 (A, E) and THP-1 (B, F) cells. C, D, G, H) IL-8 production by THP-1 (C, G) and human monocyte-derived dendritic (D, H) cells. Cells were incubated with bacteria at MOI of 1. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments using independent bacterial cultures and different MOI. *, P<0.05; **, P<0.01; ***, P<0.001. n.i., not induced.

    Article Snippet: THP-1-Blue TM cells (Invivogen), derivatives of THP-1 monocyte/macrophage human cells that stably express a NF-κB-inducible reporter system (secreted alkaline phosphatase), were added at 10 5 cells per well in 96-wells plates in HEK-Blue TM Detection medium (for NF-κB activation assay) or differentiated with 20 ng/ml of PMA for 24 h in RPMI 1640 culture medium (Lonza, Verviers, Belgium) (for IL-8 production assay).

    Techniques: Activation Assay, Derivative Assay, Incubation, Bacteria